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Bcl-2 Antibody (BH3 Domain Specific)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 32
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, IF, WB, E |
|---|---|
| Primary Accession | P10415 |
| Other Accession | P49950, O02718 |
| Reactivity | Human |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 26266 Da |
| Antigen Region | 75-110 aa |
| Gene ID | 596 |
|---|---|
| Other Names | Apoptosis regulator Bcl-2, BCL2 |
| Target/Specificity | This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-110 amino acids from human Bcl. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Bcl-2 Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BCL2 |
|---|---|
| Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1- inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). |
| Cellular Location | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. Cytoplasm {ECO:0000250|UniProtKB:P10417} |
| Tissue Location | Expressed in a variety of tissues. |
Provided below are standard protocols that you may find useful for product applications.
Background
BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.
References
Zhu, C.J., et al., J. Neurosci. Res. 74(1):60-66 (2003). Scopa, C.D., et al., Dig. Dis. Sci. 48(10):1990-1997 (2003). Grace, V.M., et al., Gynecol. Oncol. 91(1):51-58 (2003). Thoulouze, M.I., et al., Virology 314(2):549-561 (2003). Iwata, A., et al., J. Immunol. 171(6):3136-3141 (2003).
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