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POFUT2 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9Y2G5 |
| Other Accession | NP_598368.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 49976 Da |
| Antigen Region | 228-257 aa |
| Gene ID | 23275 |
|---|---|
| Other Names | GDP-fucose protein O-fucosyltransferase 2, Peptide-O-fucosyltransferase 2, O-FucT-2, POFUT2, C21orf80, FUT13, KIAA0958 |
| Target/Specificity | This POFUT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the Central region of human POFUT2. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | POFUT2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | POFUT2 |
|---|---|
| Synonyms | C21orf80, FUT13, KIAA0958 |
| Function | Catalyzes the reaction that attaches fucose through an O- glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X-X-S/T-C2 of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively (PubMed:22588082). O-fucosylates members of several protein families including the ADAMTS, the thrombospondin (TSP) and spondin families (Probable) (PubMed:17395588). Required for the proper secretion of ADAMTS family members such as ADAMTSL1 and ADAMTS13 (PubMed:17395588, PubMed:17395589). The O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm (By similarity). |
| Cellular Location | Endoplasmic reticulum. Golgi apparatus. Note=Mainly located in the endoplasmic reticulum. |
| Tissue Location | Isoform A is expressed in fetal liver and peripheral blood lymphocytes. Isoform B is expressed in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, fetal brain, fetal liver and fetal kidney. Isoform C is expressed in brain, heart, spleen, liver, lung, stomach, testis, placenta, skin, thymus, pancreas, mammary gland, prostate, fetal brain, fetal liver and fetal heart |
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Provided below are standard protocols that you may find useful for product applications.
Background
Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).
References
Feng, T., et al. Hum. Genet. 128(3):269-280(2010)
Luo, Y., et al. J. Biol. Chem. 281(14):9385-9392(2006)
Luo, Y., et al. J. Biol. Chem. 281(14):9393-9399(2006)
Menzel, O., et al. Genomics 84(2):320-330(2004)
Martinez-Duncker, I., et al. Glycobiology 13 (12), 1C-5C (2003) :
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