18,000+ products cover the ten top research categories, including cancer, metabolism, cardiovascular, neuroscience, and stem cells. Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. Quality is embedded in the production process to assure the best antibody for you.
Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications.
Abgent develops 1000+ new products per year. We provide tools for path-breaking research by developing antibodies that detect a comprehensive library of novel and established targets. For established targets we seek to add antibodies that recognize new epitopes, including post-translational modifications such as phosphorylation and methylation. For new targets we consult with leading experts to accelerate development of antibodies that will propel state-of-the-art research in cellular health and disease.
Individual peptides for SARS-CoV-2 Spike glycoprotein. In order to study the specificity of cellular immune responses against SARS CoV-2 and potential immunity caused by other human Corona Viruses, Abcepta provides Spike peptide individually, as pools and in plate. These peptides can be used for antigen specific T-cell stimulation in T-cell assays or T-cell expansion.
All peptides are manufactured in the San Diego, California. Custom peptide services include long peptides (>100 aa), cyclic peptides, difficult sequences, fluorescent labels, phospho-peptides and other post-translational modifications.
The majority of Abcepta antibodies are produced using peptide immunogens. These immuno-specific peptides can be used as blocking agents when using the complementary antibodies in a range of applications.
Abcepta's portfolio of cell lines, tissues and lysates are drawn from a range of species and immortalized cell lines. All our cell lines and lysates are validated in key applications.
These tissue lysates can be used in applications such as SDS-PAGE and Western blotting. Whole cell lysates can be used as positive controls for applications such as ELISAs, immunoprecipitation (IP) and Western blotting.
Obtaining high-quality whole cell lysates can be tedious as well as time consuming. Abcepta offers a comprehensive portfolio of whole cell lysates for research use.
Browse our catalog to find the right whole cell lysate for your experiment.
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
Abcepta is a leading manufacturer of primary antibodies with more than fifteen years of experience. We supply quality antibodies and related products drug discovery and academic laboratories worldwide. Our focus is on targets covering the major research areas impacting health and disease, including cancer, metabolism, cardiovascular, neuroscience, and stem cells.
Founded in 2001, Abcepta is one of the world's largest manufacturers of research user only antibodies. WuXi Apptec, a leading global contract R&D services provider serving the pharmaceutical, biotech, and medical device industries, acquired Abcepta in October of 2011.
With its history as an original manufacturer, Abcepta has a deep and practical understanding of the production process for antibodies, peptides, and recombinant proteins. We have assembled world-class, independently credentialed facilities, operate under a strict project management paradigm with highly trained staff, rigorously test our products in-process and post-production, and monitor customer feedback to assure that we earn the trust of scientists who choose to work with us.
Abcepta may in-licenses select products and technologies to offer our customers a wide variety of options for their proteomic research needs. Contact us for further information and non-disclosure agreements. Our experienced discovery and production teams will work with you to jointly evaluate proposals and identify partnering opportunities.
We believe that attracting and supporting creative and talented people is essential to our success. Abcepta offers an energetic, friendly environment and the opportunity to further scientific research and drug discovery. Abcepta is an Equal Opportunity Employer. Send a cover letter and resume to info@abcepta.com
Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
HIST1H2BC/HIST1H2BF Antibody (N-term) (Cat. #AP12846a) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the HIST1H2BC/HIST1H2BF antibody detected the HIST1H2BC/HIST1H2BF protein (arrow).
HIST1H2BC/HIST1H2BF Antibody (N-term) (Cat. #AP12846a) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the HIST1H2BC/HIST1H2BF antibody detected the HIST1H2BC/HIST1H2BF protein (arrow).
This HIST1H2BC/HIST1H2BF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2BC/HIST1H2BF.
Dilution
WB~~1:1000
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
HIST1H2BC/HIST1H2BF Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location
Nucleus. Chromosome.
Citations (0)
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
Submit your citation using an Abcepta antibody to info@abcepta.com, and receive a free "I Love Antibodies" mug.
Citations for related products
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Histones are basic nuclear proteins that are responsible
for the nucleosome structure of the chromosomal fiber in
eukaryotes. Nucleosomes consist of approximately 146 bp of DNA
wrapped around a histone octamer composed of pairs of each of the
four core histones (H2A, H2B, H3, and H4). The chromatin fiber is
further compacted through the interaction of a linker histone, H1,
with the DNA between the nucleosomes to form higher order chromatin
structures. This gene is intronless and encodes a member of the
histone H2B family. Transcripts from this gene lack polyA tails;
instead, they contain a palindromic termination element. This gene
is found in the large histone gene cluster on chromosome
6p22-p21.3.
References
Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)
Beck, H.C., et al. Mol. Cell Proteomics 5(7):1314-1325(2006)
Pavri, R., et al. Cell 125(4):703-717(2006)
Bonenfant, D., et al. Mol. Cell Proteomics 5(3):541-552(2006)
Siuti, N., et al. J. Proteome Res. 5(2):233-239(2006)
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