HEPACAM Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q14CZ8 |
Other Accession | NP_689935.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 46026 Da |
Antigen Region | 298-327 aa |
Gene ID | 220296 |
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Other Names | Hepatocyte cell adhesion molecule, Protein hepaCAM, HEPACAM {ECO:0000312|EMBL:AAI135631} |
Target/Specificity | This HEPACAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-327 amino acids from the C-terminal region of human HEPACAM. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | HEPACAM Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | HEPACAM {ECO:0000303|PubMed:15885354, ECO:0000312|HGNC:HGNC:26361} |
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Function | Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation (PubMed:15885354, PubMed:15917256). In glia, associates and targets CLCN2 at astrocytic processes and myelinated fiber tracts where it may regulate transcellular chloride flux involved in neuron excitability (PubMed:22405205). |
Cellular Location | Cytoplasm. Cell membrane; Single-pass type I membrane protein; Cytoplasmic side. Note=Colocalizes with CLCN2 at astrocyte end-foot in contact with brain capillaries and other glial cells (By similarity). In MCF-7 breast carcinoma and hepatic Hep 3B2.1-7 and Hep- G2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1 {ECO:0000250|UniProtKB:Q640R3} |
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Provided below are standard protocols that you may find useful for product applications.
Background
HEPACAM is involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.
References
Yang, S., et al. World J Urol 28(4):473-478(2010)
Zhang, T., et al. Int. J. Oncol. 37(1):155-165(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
He, Y., et al. BMC Cancer 10, 83 (2010) :
Lee, L.H., et al. J. Cell. Biochem. 107(6):1129-1138(2009)
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