Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
GPR34 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9UPC5 |
| Other Accession | Q9R1K6, NP_001091048.1 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 43860 Da |
| Antigen Region | 232-261 aa |
| Gene ID | 2857 |
|---|---|
| Other Names | Probable G-protein coupled receptor 34, GPR34 |
| Target/Specificity | This GPR34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-261 amino acids from the Central region of human GPR34. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GPR34 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GPR34 |
|---|---|
| Function | G-protein-coupled receptor of lysophosphatidylserine (LysoPS) that plays different roles in immune response (PubMed:16460680). Acts a damage-sensing receptor that triggers tissue repair upon recognition of dying neutrophils (By similarity). Mechanistically, apoptotic neutrophils release lysophosphatydilserine that are recognized by type 3 innate lymphoid cells (ILC3s) via GPR34, which activates downstream PI3K-AKT and RAS-ERK signaling pathways leading to STAT3 activation and IL-22 production (By similarity). Plays an important role in microglial function, controlling morphology and phagocytosis (By similarity). |
| Cellular Location | Cell membrane; Multi-pass membrane protein |
| Tissue Location | Broadly expressed. Highly expressed on mast cells (PubMed:16460680). |
Provided below are standard protocols that you may find useful for product applications.
Background
G protein-coupled receptors (GPCRs), such as GPR34, are integral membrane proteins containing 7 putative transmembrane domains (TMs). These proteins mediate signals to the interior of the cell via activation of heterotrimeric G proteins that in turn activate various effector proteins, ultimately resulting in a physiologic response.
References
Engemaier, E., et al. Genomics 87(2):254-264(2006)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Jacobi, F.K., et al. Hum. Genet. 107(1):89-91(2000)
Schoneberg, T., et al. Biochim. Biophys. Acta 1446 (1-2), 57-70 (1999) :
Marchese, A., et al. Genomics 56(1):12-21(1999)
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
