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EIF4H Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - EIF4H Antibody (Center) AP12612c
    EIF4H Antibody (Center) (Cat. #AP12612c) western blot analysis in MDA-MB231,Hela cell line lysates (35ug/lane).This demonstrates the EIF4H antibody detected the EIF4H protein (arrow).
    detail
  • IHC-P - EIF4H Antibody (Center) AP12612c
    EIF4H Antibody (Center) (Cat. #AP12612c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF4H Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q15056
Other Accession Q9WUK2, NP_114381.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 27385 Da
Antigen Region 127-156 aa
Additional Information
Gene ID 7458
Other Names Eukaryotic translation initiation factor 4H, eIF-4H, Williams-Beuren syndrome chromosomal region 1 protein, EIF4H, KIAA0038, WBSCR1, WSCR1
Target/Specificity This EIF4H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from the Central region of human EIF4H.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF4H Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF4H
Synonyms KIAA0038, WBSCR1, WSCR1
Function Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
Cellular Location Cytoplasm, perinuclear region.
Tissue Location The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney
Research Areas
Citations (0)
citation

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Background

This gene encodes one of the translation initiation factors, which functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants.

References

Feng, T., et al. Hum. Genet. 128(3):269-280(2010)
Zhu, X., et al. Genet. Epidemiol. 34(2):171-187(2010)
Mokas, S., et al. Mol. Biol. Cell 20(11):2673-2683(2009)
Marintchev, A., et al. Cell 136(3):447-460(2009)
Rozovsky, N., et al. RNA 14(10):2136-2148(2008)

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$ 182.50
$ 70.00
Cat# AP12612c
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