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APG8b(MAP1LC3B) Antibody (N-term T29)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - APG8b(MAP1LC3B) Antibody (N-term T29) AP12484a
    APG8b(MAP1LC3B) Antibody (T29) (Cat. #AP12484a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the APG8b(MAP1LC3B) antibody detected the APG8b(MAP1LC3B) protein (arrow).
    detail
  • WB - APG8b(MAP1LC3B) Antibody (N-term T29) AP12484a
    APG8b(MAP1LC3B) Antibody (T29) (Cat. #AP12484a) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the APG8b(MAP1LC3B) antibody detected the APG8b(MAP1LC3B) protein (arrow).
    detail
  • IHC-P-Leica - APG8b(MAP1LC3B) Antibody (N-term T29) AP12484a
    Immunohistochemical analysis of paraffin-embedded human brain tissue using AP12484a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P-Leica - APG8b(MAP1LC3B) Antibody (N-term T29) AP12484a
    Immunohistochemical analysis of paraffin-embedded human liver tissue using AP12484a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • FC - APG8b(MAP1LC3B) Antibody (N-term T29) AP12484a
    APG8b(MAP1LC3B) Antibody (N-term T29) (Cat. #AP12484a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P-Leica, FC, WB, E
Primary Accession Q9GZQ8
Other Accession A6NCE7, Q62625, Q9CQV6, O41515, NP_073729.1
Reactivity Human, Mouse
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 14688 Da
Antigen Region 9-33 aa
Additional Information
Gene ID 81631
Other Names Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3
Target/Specificity This APG8b(MAP1LC3B) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-33 amino acids from the N-terminal region of human APG8b(MAP1LC3B).
Dilution WB~~1:1000
IHC-P-Leica~~1:500
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPG8b(MAP1LC3B) Antibody (N-term T29) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP1LC3B (HGNC:13352)
Synonyms MAP1ALC3
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20418806, PubMed:23209295, PubMed:28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:23209295, PubMed:28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed:22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295, PubMed:28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed:24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed:30420355).
Cellular Location Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor Endomembrane system; Lipid-anchor Mitochondrion membrane; Lipid-anchor. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q9CQV6}. Cytoplasmic vesicle. Note=LC3-II binds to the autophagic membranes. LC3-II localizes with the mitochondrial inner membrane during Parkin-mediated mitophagy (PubMed:28017329). Localizes also to discrete punctae along the ciliary axoneme
Tissue Location Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver
Research Areas
Citations (0)
citation

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Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

References

Rouschop, K.M., et al. J. Clin. Invest. 120(1):127-141(2010)
Kirkin, V., et al. Mol. Cell 33(4):505-516(2009)
Othman, E.Q., et al. J. Clin. Lab. Anal. 23(4):249-258(2009)
Liu, Q., et al. Ai Zheng 27(1):25-29(2008)
Komatsu, M., et al. Cell 131(6):1149-1163(2007)

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$ 365.00
$ 140.00
Cat# AP12484a
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