FASTK Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | Q14296 |
Other Accession | NP_006703.1, NP_148936.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 61104 Da |
Antigen Region | 395-424 aa |
Gene ID | 10922 |
---|---|
Other Names | Fas-activated serine/threonine kinase, FAST kinase, FASTK |
Target/Specificity | This FASTK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-424 amino acids from the C-terminal region of human FASTK. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | FASTK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | FASTK |
---|---|
Function | Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro- apoptotic form of the receptor. |
Cellular Location | [Isoform 4]: Mitochondrion matrix. Note=Colocalizes with mitochondrial RNA granules. |
Tissue Location | Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined.
References
Simarro, M., et al. J. Immunol. 184(9):5325-5332(2010)
de Krom, M., et al. Biol. Psychiatry 65(7):625-630(2009)
Simarro, M., et al. Proc. Natl. Acad. Sci. U.S.A. 104(27):11370-11375(2007)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Izquierdo, J.M., et al. J. Biol. Chem. 282(3):1539-1543(2007)
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