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POLR2G Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - POLR2G Antibody (C-term) AP12458b
    POLR2G Antibody (C-term) (Cat. #AP12458b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the POLR2G antibody detected the POLR2G protein (arrow).
    detail
  • FC - POLR2G Antibody (C-term) AP12458b
    POLR2G Antibody (C-term) (Cat. #AP12458b) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P62487
Other Accession P62489, P62488, Q7ZW41, Q5E9B8, NP_002687.1
Reactivity Human
Predicted Bovine, Zebrafish, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 19294 Da
Antigen Region 126-153 aa
Additional Information
Gene ID 5436
Other Names DNA-directed RNA polymerase II subunit RPB7, RNA polymerase II subunit B7, DNA-directed RNA polymerase II subunit G, RNA polymerase II 19 kDa subunit, RPB19, POLR2G, RPB7
Target/Specificity This POLR2G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-153 amino acids from the C-terminal region of human POLR2G.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLR2G Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLR2G
Synonyms RPB7
Function Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2G/RPB7 is part of a subcomplex with POLR2D/RPB4 that binds to a pocket formed by POLR2A/RPB1, POLR2B/RPB2 and POLR2F/RPABC2 at the base of the clamp element. The POLR2D/RPB4- POLR2G/RPB7 subcomplex seems to lock the clamp via POLR2G/RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The POLR2D/RPB4-POLR2G/RPB7 subcomplex binds single- stranded DNA and RNA.
Cellular Location Nucleus.
Research Areas
Citations (0)
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Background

This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.

References

Cojocaru, M., et al. Biochem. J. 409(1):139-147(2008)
Ujvari, A., et al. Nat. Struct. Mol. Biol. 13(1):49-54(2006)
Meka, H., et al. Nucleic Acids Res. 33(19):6435-6444(2005)
Lehner, B., et al. Genomics 83(1):153-167(2004)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671(2003)

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$ 365.00
$ 140.00
Cat# AP12458b
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