IGSF8 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
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Primary Accession | Q969P0 |
Other Accession | NP_443100.1 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 65034 Da |
Antigen Region | 226-254 aa |
Gene ID | 93185 |
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Other Names | Immunoglobulin superfamily member 8, IgSF8, CD81 partner 3, Glu-Trp-Ile EWI motif-containing protein 2, EWI-2, Keratinocytes-associated transmembrane protein 4, KCT-4, LIR-D1, Prostaglandin regulatory-like protein, PGRL, CD316, IGSF8, CD81P3, EWI2, KCT4 |
Target/Specificity | This IGSF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-254 amino acids from the Central region of human IGSF8. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | IGSF8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | IGSF8 |
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Synonyms | CD81P3, EWI2, KCT4 |
Function | Member of the immunoglobulin superfamily (IgSF) that links tetraspanin-enriched microdomains to the actin cytoskeleton and plays several important roles in innate and adaptive immunity (PubMed:11504738, PubMed:14662754). Acts as an inducible receptor of HSPA8 on dendritic cells to enhance the CCL21/SLC-dependent migration of activated mature dendritic cells while attenuating their antigen- specific stimulatory capacities (PubMed:17785435). In complex with alpha-actinins ACTN1 and ACTN4, regulates actin dynamics in the immune synapse and subsequent T-cell activation (PubMed:22689882). Inhibits the entry of several viruses such as hepatitis C Virus (HCV) or HIV-1. Mechanistically, promotes a change in CD81 organization at the plasma membrane by significantly restricting its diffusion which in turn influences CD81 interaction with Claudin-1/CLDN1, preventing CLDN1 from acting as a co-receptor required for HCV entry (PubMed:23351194). Accumulates at the presynaptic terminal, the producer cell side of the virological synapse, to prevent HIV-1 Env-mediated cell-cell fusion (PubMed:31757023). Highly expressed on malignant cells with antigen presentation defects, interacts with NK receptor KIR3DL2 to suppress NK-cell cytotoxicity (PubMed:38657602). May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain. |
Cellular Location | Cell membrane; Single-pass membrane protein. Note=Colocalizes with CD81 at the immune synapse. |
Tissue Location | Expressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. Detected on a majority of B-cells, T-cells, and natural killer cells (PubMed:12708969). Expressed on dendritic cells (PubMed:17785435) |
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Provided below are standard protocols that you may find useful for product applications.
Background
IGSF8 may play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.
References
Kolesnikova, T.V., et al. Neoplasia 11(1):77-86(2009)
Rocha-Perugini, V., et al. PLoS ONE 3 (4), E1866 (2008) :
Kettner, S., et al. Mol. Cell. Biol. 27(21):7718-7726(2007)
Sala-Valdes, M., et al. J. Biol. Chem. 281(28):19665-19675(2006)
Yang, X.H., et al. J. Biol. Chem. 281(18):12976-12985(2006)
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