CKAP4 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | Q07065 |
Other Accession | NP_006816.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 66022 Da |
Antigen Region | 574-602 aa |
Gene ID | 10970 |
---|---|
Other Names | Cytoskeleton-associated protein 4, 63-kDa cytoskeleton-linking membrane protein, Climp-63, p63, CKAP4 |
Target/Specificity | This CKAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 574-602 amino acids from the C-terminal region of human CKAP4. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | CKAP4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CKAP4 |
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Function | Mediates the anchoring of the endoplasmic reticulum to microtubules. |
Cellular Location | Endoplasmic reticulum membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region Note=Translocates to the perinuclear region upon APF-stimulation |
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Provided below are standard protocols that you may find useful for product applications.
Background
CKAP4 is a substrate of DHHC2, a putative tumor suppressor. CK MNF116 and CKAP4 were useful in identifying squamous cell carcinomas with single cell infiltration. These results suggest a role for CKAP4 in tumor.
References
Kanner, W.A., et al. J. Cutan. Pathol. 37(7):744-750(2010)
Tomkova, H., et al. Eur J Dermatol 20(3):411-413(2010)
Lefkimmiatis, K., et al. Cancer Res. 69(22):8563-8571(2009)
Planey, S.L., et al. Mol. Biol. Cell 20(5):1454-1463(2009)
Bates, S.R., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 295 (4), L658-L669 (2008) :
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Anonymous
2016-08-23 21:15:32
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Species tested
Human
Application tested
WB
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature.
2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃
3. Wash 5*5 min with TBST.
4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature.
5. Wash 5*5 min with TBST.
6. Incubate with ECL substrates and expose |