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NEDD8 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q15843 |
| Other Accession | Q71UE8, Q4PLJ0, P29595, P61282 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rabbit, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 9072 Da |
| Antigen Region | 1-32 aa |
| Gene ID | 4738 |
|---|---|
| Other Names | NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8, Ubiquitin-like protein Nedd8, NEDD8 |
| Target/Specificity | This NEDD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-32 amino acids from the N-terminal region of human NEDD8. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NEDD8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NEDD8 {ECO:0000303|PubMed:9694792, ECO:0000312|HGNC:HGNC:7732} |
|---|---|
| Function | Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53 (PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:14690597, PubMed:15242646, PubMed:9694792, PubMed:38605244). Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins (PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:20688984, PubMed:9694792, PubMed:38605244). Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity (PubMed:15242646). Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M (PubMed:14690597). |
| Cellular Location | Nucleus. Note=Mainly nuclear. |
| Tissue Location | Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes |
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Provided below are standard protocols that you may find useful for product applications.
Background
NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
References
Wu, K., et al., J. Biol. Chem. 278(31):28882-28891 (2003).
Bohnsack, R.N., et al., J. Biol. Chem. 278(29):26823-26830 (2003).
Fan, M., et al., Mol. Endocrinol. 17(3):356-365 (2003).
Walden, H., et al., Nature 422(6929):330-334 (2003).
Liu, J., et al., Mol. Cell 10(6):1511-1518 (2002).
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