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SNIP1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SNIP1 Antibody (N-term) AP12249A
    SNIP1 Antibody (N-term) (Cat. #AP12249a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SNIP1 antibody detected the SNIP1 protein (arrow).
    detail
  • FC - SNIP1 Antibody (N-term) AP12249A
    SNIP1 Antibody (N-term) (Cat. #AP12249a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q8TAD8
Other Accession Q5M9G6, Q8BIZ6, NP_078976.2
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 45778 Da
Antigen Region 91-118 aa
Additional Information
Gene ID 79753
Other Names Smad nuclear-interacting protein 1, FHA domain-containing protein SNIP1, SNIP1
Target/Specificity This SNIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-118 amino acids from the N-terminal region of human SNIP1.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSNIP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SNIP1
Function Required for pre-mRNA splicing as component of the spliceosome (PubMed:29360106). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. Involved in the microRNA (miRNA) biogenesis. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.
Cellular Location Nucleus
Tissue Location Ubiquitous, with highest expression in heart and skeletal muscle.
Research Areas
Citations (0)
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Background

Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. SNIP1 is involved in the microRNA (miRNA) biogenesis.

References

Bracken, C.P., et al. Cancer Res. 68(18):7621-7628(2008)
Yu, B., et al. Proc. Natl. Acad. Sci. U.S.A. 105(29):10073-10078(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)

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$ 182.50
$ 70.00
Cat# AP12249A
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