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PYGO1 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - PYGO1 Antibody (C-term) AP12187b
    Confocal immunofluorescent analysis of PYGO1 Antibody (C-term)(Cat#AP12187b) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - PYGO1 Antibody (C-term) AP12187b
    PYGO1 Antibody (C-term) (Cat. #AP12187b) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PYGO1 antibody detected the PYGO1 protein (arrow).
    detail
  • IHC-P - PYGO1 Antibody (C-term) AP12187b
    PYGO1 Antibody (C-term) (Cat. #AP12187b)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PYGO1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, IHC-P, WB, E
Primary Accession Q9Y3Y4
Other Accession NP_056432.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 45116 Da
Antigen Region 382-409 aa
Additional Information
Gene ID 26108
Other Names Pygopus homolog 1, PYGO1
Target/Specificity This PYGO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-409 amino acids from the C-terminal region of human PYGO1.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPYGO1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PYGO1
Function Involved in signal transduction through the Wnt pathway.
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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Background

PYGO1 is involved in signal transduction through the Wnt pathway.

References

Frank, B., et al. Carcinogenesis (2010) In press :
Fiedler, M., et al. Mol. Cell 30(4):507-518(2008)
Townsley, F.M., et al. J. Biol. Chem. 279(7):5177-5183(2004)
Thompson, B., et al. Nat. Cell Biol. 4(5):367-373(2002)
Kramps, T., et al. Cell 109(1):47-60(2002)

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$ 182.50
$ 70.00
Cat# AP12187b
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