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KLF4 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O43474 |
| Other Accession | NP_004226.3 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 54671 Da |
| Antigen Region | 283-314 aa |
| Gene ID | 9314 |
|---|---|
| Other Names | Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4, EZF, GKLF |
| Target/Specificity | This KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-314 amino acids from the Central region of human KLF4. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | KLF4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | KLF4 (HGNC:6348) |
|---|---|
| Synonyms | EZF, GKLF |
| Function | Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:Q60793}. Cytoplasm {ECO:0000250|UniProtKB:Q60793} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
KLF4 is a transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.
References
Guan, H., et al. Blood 116(9):1469-1478(2010)
Wong, C.W., et al. Stem Cells 28(9):1510-1517(2010)
Ohnesorge, N., et al. J. Biol. Chem. 285(34):26199-26210(2010)
Cekanova, M., et al. Eur. J. Pharmacol. 637 (1-3), 30-37 (2010) :
Yori, J.L., et al. J. Biol. Chem. 285(22):16854-16863(2010)
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