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RBPJL Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RBPJL Antibody (N-term) AP12042a
    Anti-RBPJL Antibody (N-term) at 1:2000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - RBPJL Antibody (N-term) AP12042a
    Anti-RBPJL Antibody (N-term) at 1:2000 dilution + HUVEC whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - RBPJL Antibody (N-term) AP12042a
    RBPJL Antibody (N-term) (Cat. #AP12042a)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBPJL Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - RBPJL Antibody (N-term) AP12042a
    RBPJL Antibody (N-term) (Cat. #AP12042a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession Q9UBG7
Other Accession NP_055091.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 56751 Da
Antigen Region 8-36 aa
Additional Information
Gene ID 11317
Other Names Recombining binding protein suppressor of hairless-like protein, Transcription factor RBP-L, RBPJL, RBPL, RBPSUHL
Target/Specificity This RBPJL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-36 amino acids from the N-terminal region of human RBPJL.
Dilution WB~~1:2000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRBPJL Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RBPJL
Synonyms RBPL, RBPSUHL
Function Putative transcription factor, which cooperates with EBNA2 to activate transcription.
Cellular Location Nucleus.
Citations (0)
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Background

In mouse, recombining binding protein L (RBP-L) is a transcription factor that binds to DNA sequences almost identical to that bound by the Notch receptor signalling pathway transcription factor RBP-J. However, unlike RBP-J, RBP-L does not interact with Notch receptors. RBP-L has been shown to activate transcription in concert with Epstein-Barr virus nuclear antigen-2 (EBNA2). The protein encoded by this gene is similar in sequence to the mouse RPB-L protein and Drosophila suppressor of hairless protein.

References

Beres, T.M., et al. Mol. Cell. Biol. 26(1):117-130(2006)
Deloukas, P., et al. Nature 414(6866):865-871(2001)
Nakagawa, H., et al. Oncogene 19(2):210-216(2000)
Tani, S., et al. J. Hum. Genet. 44(1):73-75(1999)
Minoguchi, S., et al. Mol. Cell. Biol. 17(5):2679-2687(1997)

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$ 182.50
$ 70.00
Cat# AP12042a
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