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TFAP4 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - TFAP4 Antibody (Center) AP11969c
    TFAP4 Antibody (Center) (Cat. #AP11969c) western blot analysis in Hela,HL-60 cell line lysates (35ug/lane).This demonstrates the TFAP4 antibody detected the TFAP4 protein (arrow).
    detail
  • FC - TFAP4 Antibody (Center) AP11969c
    TFAP4 Antibody (Center) (Cat. #AP11969c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q01664
Other Accession NP_003214
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 38726 Da
Antigen Region 125-154 aa
Additional Information
Gene ID 7023
Other Names Transcription factor AP-4, Activating enhancer-binding protein 4, Class C basic helix-loop-helix protein 41, bHLHc41, TFAP4, BHLHC41
Target/Specificity This TFAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the Central region of human TFAP4.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTFAP4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TFAP4
Synonyms BHLHC41
Function Transcription factor that activates both viral and cellular genes by binding to the symmetrical DNA sequence 5'-CAGCTG-3'.
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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Background

Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).

References

Ku, W.C., et al. Mol. Cell Proteomics 8(9):2034-2050(2009)
Cao, J., et al. Int. J. Surg. Pathol. 17(1):16-21(2009)
Jung, P., et al. Proc. Natl. Acad. Sci. U.S.A. 105(39):15046-15051(2008)
Pons, J., et al. Biochemistry 47(1):14-29(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

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$ 365.00
$ 140.00
Cat# AP11969c
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