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WT1 Antibody (Center E361)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, IHC-P, FC, WB, E |
|---|---|
| Primary Accession | P19544 |
| Other Accession | P49952, O62651, P22561, P79958, B7ZSG3, NP_000369 |
| Reactivity | Human, Mouse |
| Predicted | Xenopus, Pig, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 49188 Da |
| Antigen Region | 346-375 aa |
| Gene ID | 7490 |
|---|---|
| Other Names | Wilms tumor protein, WT33, WT1 |
| Target/Specificity | This WT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the Central region of human WT1. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:50~100 FC~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | WT1 Antibody (Center E361) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | WT1 |
|---|---|
| Function | Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:17716689, PubMed:25258363, PubMed:7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921). |
| Cellular Location | Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Isoforms lacking the KTS motif have a diffuse nuclear location (PubMed:15520190). Shuttles between nucleus and cytoplasm. {ECO:0000250, ECO:0000269|PubMed:15520190} [Isoform 4]: Nucleus, nucleoplasm |
| Tissue Location | Expressed in the kidney and a subset of hematopoietic cells |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].
References
Sitaram, R.T., et al. Br. J. Cancer 103(8):1255-1262(2010)
Dohi, S., et al. Anticancer Res. 30(8):3187-3192(2010)
Rocquain, J., et al. BMC Cancer 10, 401 (2010) :
Wagner, K.D., et al. J. Cell. Sci. 116 (PT 9), 1653-1658 (2003) :
Mitsuya, K., et al. Hum. Mol. Genet. 6(13):2243-2246(1997)
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