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WT1 Antibody (Center E361)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| IF, IHC-P, FC, WB, E |
|---|---|
| Primary Accession | P19544 |
| Other Accession | P49952, O62651, P22561, P79958, B7ZSG3, NP_000369 |
| Reactivity | Human, Mouse |
| Predicted | Xenopus, Pig, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 49188 Da |
| Antigen Region | 346-375 aa |
| Gene ID | 7490 |
|---|---|
| Other Names | Wilms tumor protein, WT33, WT1 |
| Target/Specificity | This WT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the Central region of human WT1. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:50~100 FC~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | WT1 Antibody (Center E361) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | WT1 |
|---|---|
| Function | Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:17716689, PubMed:25258363, PubMed:7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921). |
| Cellular Location | Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Isoforms lacking the KTS motif have a diffuse nuclear location (PubMed:15520190). Shuttles between nucleus and cytoplasm. {ECO:0000250, ECO:0000269|PubMed:15520190} [Isoform 4]: Nucleus, nucleoplasm |
| Tissue Location | Expressed in the kidney and a subset of hematopoietic cells |
Research Areas
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$ 192.50
Cat# AP11964c
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