RAP1GDS1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | P52306 |
Other Accession | NP_001093900.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 66317 Da |
Antigen Region | 217-244 aa |
Gene ID | 5910 |
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Other Names | Rap1 GTPase-GDP dissociation stimulator 1, Exchange factor smgGDS, SMG GDS protein, SMG P21 stimulatory GDP/GTP exchange protein, RAP1GDS1 |
Target/Specificity | This RAP1GDS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-244 amino acids from the Central region of human RAP1GDS1. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | RAP1GDS1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RAP1GDS1 {ECO:0000303|Ref.3} |
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Synonyms | SMGGDS {ECO:0000303|Ref.3} |
Function | Acts as a GEF (guanine nucleotide exchange factor) for the Rho family of small GTP-binding proteins (G proteins) that stimulates the dissociation of GDP to enable subsequent binding of GTP (PubMed:11948427, PubMed:1549351, PubMed:20709748, PubMed:28630045, PubMed:30190425). Additionally, appears to chaperone the processing and/or trafficking of small GTPases containing a C-terminal polybasic region independently of GEF activity (PubMed:20709748, PubMed:21242305). Targets include RAP1A/RAP1B, RHOA, RHOB, RHOC, RAC1 and KRAS (PubMed:11948427, PubMed:1549351, PubMed:20709748, PubMed:24415755). Regulates mitochondrial dynamics by controlling RHOT function to promote mitochondrial fission during high calcium conditions (PubMed:27716788). Able to promote the Ca(2+) release from the endoplasmic reticulum via both inositol trisphosphate (Ins3P) and ryanodine sensitive receptors leading to a enhanced mitochondrial Ca(2+) uptake (PubMed:24349085). |
Cellular Location | Cytoplasm, cytosol. Endoplasmic reticulum. Mitochondrion. Nucleus Note=Nuclear import is dependent on complexing with a GTPase containing a C-terminal polybasic region. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The smg GDP dissociation stimulator (smgGDS) protein is a stimulatory GDP/GTP exchange protein with GTPase activity (Riess et al., 1993 [PubMed 8262526]).
References
Schonherr, C., et al. Oncogene 29(19):2817-2830(2010)
Branham, M.T., et al. J. Biol. Chem. 284(37):24825-24839(2009)
Zemunik, T., et al. Croat. Med. J. 50(1):23-33(2009)
Tew, G.W., et al. J. Biol. Chem. 283(2):963-976(2008)
Tiwari, S., et al. Blood 103(7):2661-2667(2004)
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