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TUBB6 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - TUBB6 Antibody (Center) AP11928c
    All lanes : Anti-TUBB6 Antibody (Center) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: A431 whole cell lysate Lane 5: A549 whole cell lysate Lane 6: U-251MG whole cell lysate Lane 7: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9BUF5
Other Accession Q9D6F9, Q4R4X8, P04350, Q3ZBU7, NP_115914.1
Reactivity Human
Predicted Bovine, Monkey, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 49857 Da
Antigen Region 139-166 aa
Additional Information
Gene ID 84617
Other Names Tubulin beta-6 chain, Tubulin beta class V, TUBB6
Target/Specificity This TUBB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-166 amino acids from the Central region of human TUBB6.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTUBB6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBB6
Function Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Ubiquitous. Maximal expression in breast and lung, where it represents around 10% of all beta-tubulins. Largely decreased expression in most cancerous tissues.
Research Areas
Citations (0)
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Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).

References

Leandro-Garcia, L.J., et al. Cytoskeleton (Hoboken) 67(4):214-223(2010)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)

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$ 365.00
$ 140.00
Cat# AP11928c
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