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NKX1-1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - NKX1-1 Antibody (Center) AP11916c
    Fluorescent confocal image of Hela cell stained with NKX1-1 Antibody (Center)(Cat#AP11916c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NKX1-1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NKX1-1 immunoreactivity is localized to Nucleus significantly.
    detail
  • WB - NKX1-1 Antibody (Center) AP11916c
    NKX1-1 Antibody (Center) (Cat. #AP11916c) western blot analysis in SK-N-MC cell line lysates (35ug/lane).This demonstrates the NKX1-1 antibody detected the NKX1-1 protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q15270
Other Accession XP_931434.4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 44012 Da
Antigen Region 1-1 aa
Additional Information
Gene ID 54729
Other Names NK1 transcription factor-related protein 1, Homeobox protein 153, HPX-153, Homeobox protein SAX-2, NKX-11, NKX1-1, HPX153
Target/Specificity This NKX1-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-258 amino acids from the Central region of human NKX1-1.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNKX1-1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NKX1-1 (HGNC:24975)
Function May be required for the coordinated crosstalk of factors involved in the maintenance of energy homeostasis, possibly by regulating the transcription of specific factors involved in energy balance.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
Tissue Location Expressed in hemopoietic progenitor cells.
Research Areas
Citations (0)
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Background

The function of this protein remains unknown.

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$ 365.00
$ 140.00
Cat# AP11916c
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