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TGFBR2 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - TGFBR2 Antibody (N-term) AP11854A
    Confocal immunofluorescent analysis of TGFBR2 Antibody (N-term) (Cat#AP11854a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - TGFBR2 Antibody (N-term) AP11854A
    All lanes : Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution Lane 1: NCCIT whole cell lysates Lane 2: mouse lung lysates Lane 3: SW620 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - TGFBR2 Antibody (N-term) AP11854A
    Anti-TGFBR2 Antibody (N-term)at 1:2000 dilution + mouse lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - TGFBR2 Antibody (N-term) AP11854A
    All lanes : Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution Lane 1: human testis lysate Lane 2: mouse lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - TGFBR2 Antibody (N-term) AP11854A
    Anti-TGFBR2 Antibody (N-term)at 1:2000 dilution + mouse lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - TGFBR2 Antibody (N-term) AP11854A
    TGFBR2 Antibody (N-term) (Cat. #AP11854a) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the TGFBR2 antibody detected the TGFBR2 protein (arrow).
    detail
  • WB - TGFBR2 Antibody (N-term) AP11854A
    Anti-TGFBR2 Antibody (N-term)at 1:2000 dilution + mouse lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - TGFBR2 Antibody (N-term) AP11854A
    Anti-TGFBR2 Antibody (N-term)at 1:1000 dilution + mouse lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - TGFBR2 Antibody (N-term) AP11854A
    AP11854a staining TGFBR2 in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • IHC-P - TGFBR2 Antibody (N-term) AP11854A
    AP11854a staining TGFBR2 in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • IHC-P - TGFBR2 Antibody (N-term) AP11854A
    TGFBR2 Antibody (N-term) (Cat. #AP11854a)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TGFBR2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • IHC-P - TGFBR2 Antibody (N-term) AP11854A
    AP11854a staining TGFBR2 in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P37173
Other Accession NP_003233.4, NP_001020018.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 64568 Da
Antigen Region 13-40 aa
Additional Information
Gene ID 7048
Other Names TGF-beta receptor type-2, TGFR-2, TGF-beta type II receptor, Transforming growth factor-beta receptor type II, TGF-beta receptor type II, TbetaR-II, TGFBR2
Target/Specificity This TGFBR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-40 amino acids from the N-terminal region of human TGFBR2.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTGFBR2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TGFBR2
Function Transmembrane serine/threonine kinase forming with the TGF- beta type I serine/threonine kinase receptor, TGFBR1, the non- promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and thus regulates a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways.
Cellular Location Cell membrane; Single-pass type I membrane protein. Membrane raft
Research Areas
Citations ( 0 )

Background

This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized.

References

Inamoto, S., et al. Cardiovasc. Res. 88(3):520-529(2010)
Bianchini, G., et al. J. Clin. Oncol. 28(28):4316-4323(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Kim, J.N., et al. Toxicology 275 (1-3), 29-35 (2010) :
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :

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$ 365.00
$ 140.00
Cat# AP11854A
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