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CCDC36 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - CCDC36 Antibody (C-term) AP11750b
    Confocal immunofluorescent analysis of CCDC36 Antibody (C-term)(Cat#AP11750b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - CCDC36 Antibody (C-term) AP11750b
    Western blot analysis of lysates from 293,Neuro-2a cell line and mouse liver tissue (from left to right),using CCDC36 Antibody (C-term)(Cat. #AP11750b).AP11750b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
    detail
  • IHC-P - CCDC36 Antibody (C-term) AP11750b
    CCDC36 Antibody (C-term) (Cat. #AP11750b)immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCDC36 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P, E
Primary Accession Q8IYA8
Other Accession NP_001128669.1, NP_835467.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 66343 Da
Antigen Region 422-450 aa
Additional Information
Gene ID 339834
Other Names Coiled-coil domain-containing protein 36, Cancer/testis antigen 74, CT74, CCDC36
Target/Specificity This CCDC36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 422-450 amino acids from the C-terminal region of human CCDC36.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCDC36 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IHO1 {ECO:0000303|PubMed:27723721, ECO:0000312|HGNC:HGNC:27945}
Function Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.
Cellular Location Chromosome {ECO:0000250|UniProtKB:Q6PDM4}. Note=Specifically localizes to unsynapsed chromosomal regions during meiosis. {ECO:0000250|UniProtKB:Q6PDM4}
Research Areas
Citations (0)
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References

Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002)

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$ 365.00
$ 140.00
Cat# AP11750b
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