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BTC Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - BTC Antibody (N-term) AP11669a
    Confocal immunofluorescent analysis of BTC Antibody (N-term)(Cat#AP11669a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - BTC Antibody (N-term) AP11669a
    BTC Antibody (N-term) (Cat. #AP11669a) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the BTC antibody detected the BTC protein (arrow).
    detail
  • IHC-P - BTC Antibody (N-term) AP11669a
    BTC Antibody (N-term) (Cat. #AP11669a)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BTC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - BTC Antibody (N-term) AP11669a
    BTC Antibody (N-term) (Cat. #AP11669a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, FC, IHC-P, WB, E
Primary Accession P35070
Other Accession NP_001720.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 19746 Da
Antigen Region 17-45 aa
Additional Information
Gene ID 685
Other Names Probetacellulin, Betacellulin, BTC, BTC
Target/Specificity This BTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human BTC.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBTC Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BTC
Function Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Cellular Location [Betacellulin]: Secreted, extracellular space.
Tissue Location Synthesized in several tissues and tumor cells. Predominantly expressed in pancreas and small intestine
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor.

References

Stoeck, A., et al. J. Cell. Sci. 123 (PT 13), 2319-2331 (2010) :
Genetos, D.C., et al. Cell Tissue Res. 340(1):81-89(2010)
Nagaoka, T., et al. J. Mol. Biol. 380(1):83-94(2008)
Revillion, F., et al. Ann. Oncol. 19(1):73-80(2008)
Moss, M.L., et al. J. Biol. Chem. 282(49):35712-35721(2007)

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$ 182.50
$ 70.00
Cat# AP11669a
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