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OPN-a/b Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 6
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | P10451 |
| Other Accession | NP_000573.1, NP_001035147.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 35423 Da |
| Antigen Region | 14-40 aa |
| Gene ID | 6696 |
|---|---|
| Other Names | Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, SPP1, BNSP, OPN |
| Target/Specificity | This OPN-a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-40 amino acids from the N-terminal region of human OPN-a/b. |
| Dilution | IF~~1:25 WB~~1:2000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | OPN-a/b Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SPP1 |
|---|---|
| Synonyms | BNSP, OPN |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. |
| Cellular Location | Secreted |
| Tissue Location | Detected in cerebrospinal fluid and urine (at protein level) (PubMed:25326458, PubMed:36213313, PubMed:37453717) Bone. Found in plasma. |
Provided below are standard protocols that you may find useful for product applications.
Background
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Weber, G.F., et al. Br. J. Cancer 103(6):861-869(2010)
Chen, R.X., et al. J. Gastroenterol. Hepatol. 25(8):1435-1442(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
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