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PIK3R5 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
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- BACKGROUND
Application 
| WB, IHC-P, FC, E |
|---|---|
| Primary Accession | Q8WYR1 |
| Other Accession | NP_001136105.1, NP_055123.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 97348 Da |
| Antigen Region | 763-792 aa |
| Gene ID | 23533 |
|---|---|
| Other Names | Phosphoinositide 3-kinase regulatory subunit 5, PI3-kinase regulatory subunit 5, PI3-kinase p101 subunit, Phosphatidylinositol 4, 5-bisphosphate 3-kinase regulatory subunit, PtdIns-3-kinase regulatory subunit, Protein FOAP-2, PtdIns-3-kinase p101, p101-PI3K, PIK3R5 |
| Target/Specificity | This PIK3R5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 763-792 amino acids from the C-terminal region of human PIK3R5. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PIK3R5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PIK3R5 |
|---|---|
| Function | Regulatory subunit of the PI3K gamma complex. Required for recruitment of the catalytic subunit to the plasma membrane via interaction with beta-gamma G protein dimers. Required for G protein- mediated activation of PIK3CG (By similarity). |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:O02696}. Cytoplasm {ECO:0000250|UniProtKB:O02696}. Cell membrane {ECO:0000250|UniProtKB:O02696}; Peripheral membrane protein {ECO:0000250|UniProtKB:O02696}. Note=Predominantly localized in the nucleus in absence of PIK3CG/p120. Colocalizes with PIK3CG/p120 in the cytoplasm. Translocated to the plasma membrane in a beta-gamma G protein-dependent manner. {ECO:0000250|UniProtKB:O02696} |
| Tissue Location | Ubiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Receptor-regulated class I phosphoinositide 3-kinases (PI3Ks) phosphorylate the membrane lipid phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to PtdIns(3,4,5)P3, which in turn recruits and activates cytosolic effectors involved in proliferation, survival, or chemotaxis. PIK3R5 is a PI3K regulatory subunit (Brock et al., 2003 [PubMed 12507995]).
References
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Segat, L., et al. Vaccine 28(10):2201-2206(2010)
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Johnson, C., et al. Oncogene 26(49):7049-7057(2007)
Suire, S., et al. Curr. Biol. 15(6):566-570(2005)
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