COPE Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF, IHC-P, E |
---|---|
Primary Accession | O14579 |
Other Accession | O89079, Q60445, Q5ZIK9, NP_009194.2 |
Reactivity | Human |
Predicted | Chicken, Hamster, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 34482 Da |
Antigen Region | 273-300 aa |
Gene ID | 11316 |
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Other Names | Coatomer subunit epsilon, Epsilon-coat protein, Epsilon-COP, COPE |
Target/Specificity | This COPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-300 amino acids from the C-terminal region of human COPE. |
Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | COPE Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | COPE |
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Function | The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin- coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). |
Cellular Location | Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
References
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Morikawa, R.K., et al. J. Biol. Chem. 284(39):26620-26630(2009)
Maruyama, S., et al. Mol. Cell. Biochem. 307 (1-2), 73-82 (2008) :
Goryachev, A.B., et al. PLoS Comput. Biol. 2 (12), E172 (2006) :
Lippincott-Schwartz, J., et al. Trends Cell Biol. 16 (10), E1-E4 (2006) :
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