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PIP5KL1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - PIP5KL1 Antibody (N-term) AP11297a
    Confocal immunofluorescent analysis of PIP5KL1 Antibody (N-term)(Cat#AP11297a) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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  • WB - PIP5KL1 Antibody (N-term) AP11297a
    Western blot analysis of lysates from 293,K562,PC-3 cell line (from left to right),using PIP5KL1 Antibody (N-term)(Cat. #AP11297a).AP11297a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
    detail
  • IHC-P - PIP5KL1 Antibody (N-term) AP11297a
    PIP5KL1 Antibody (N-term) (Cat. #AP11297a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIP5KL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P, E
Primary Accession Q5T9C9
Other Accession NP_001128691.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 44572 Da
Antigen Region 57-86 aa
Additional Information
Gene ID 138429
Other Names Phosphatidylinositol 4-phosphate 5-kinase-like protein 1, PI(4)P 5-kinase-like protein 1, PtdIns(4)P-5-kinase-like protein 1, PIP5KL1
Target/Specificity This PIP5KL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 amino acids from the N-terminal region of human PIP5KL1.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPIP5KL1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIP5KL1
Function May act as a scaffold to localize and regulate type I PI(4)P 5-kinases to specific compartments within the cell, where they generate PI(4,5)P2 for actin nucleation, signaling and scaffold protein recruitment and conversion to PI(3,4,5)P3.
Cellular Location Cytoplasm. Membrane. Note=Localized to large cytoplasmic vesicular structures.
Research Areas
Citations (0)
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Background

PIP5KL1 is a phosphoinositide kinase-like protein that lacks intrinsic lipid kinase activity but associates with type I PIPKs (see PIP5K1A; MIM 603275) and may play a role in localization of PIPK activity (Chang et al., 2004 [PubMed 14701839]).[supplied by OMIM].

References

Shi, L., et al. Mol. Biol. Rep. 37(5):2189-2198(2010)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Chang, J.D., et al. J. Biol. Chem. 279(12):11672-11679(2004)

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$ 365.00
$ 140.00
Cat# AP11297a
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