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FUT8 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - FUT8 Antibody (Center) AP11123c
    FUT8 Antibody (Center) (Cat. #AP11123c) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FUT8 antibody detected the FUT8 protein (arrow).
    detail
  • WB - FUT8 Antibody (Center) AP11123c
    Western blot analysis of lysate from Hela cell line, using FUT8 Antibody (Center)(Cat. #AP11123c). AP11123c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
    detail
  • IHC-P - FUT8 Antibody (Center) AP11123c
    FUT8 Antibody (Center) (Cat. #AP11123c)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FUT8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9BYC5
Other Accession Q6EV76, P79282, Q9WTS2, Q9N0W2, NP_835367.1, NP_835369.1
Reactivity Human
Predicted Bovine, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 66516 Da
Antigen Region 329-357 aa
Additional Information
Gene ID 2530
Other Names Alpha-(1, 6)-fucosyltransferase, Alpha1-6FucT, Fucosyltransferase 8, GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1, 6-fucosyltransferase, GDP-fucose--glycoprotein fucosyltransferase, Glycoprotein 6-alpha-L-fucosyltransferase, FUT8
Target/Specificity This FUT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-357 amino acids from the Central region of human FUT8.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFUT8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FUT8
Function Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.
Cellular Location Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein Note=Membrane-bound form in trans cisternae of Golgi.
Research Areas
Citations (0)
citation

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Background

This enzyme belongs to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternatively spliced variants encoding different isoforms have been identified.

References

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Wang, X., et al. J. Biochem. 145(5):643-651(2009)
Kudo, T., et al. Mol. Cancer 6, 32 (2007) :
Ihara, H., et al. Glycobiology 16(4):333-342(2006)
Ito, Y., et al. Cancer Lett. 200(2):167-172(2003)

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$ 365.00
$ 140.00
Cat# AP11123c
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