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His6-DEP-1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - His6-DEP-1 Antibody AP11085A
    Confocal immunofluorescent analysis of His6-DEP-1 Antibody (Cat. #AP11085a) with Hela cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - His6-DEP-1 Antibody AP11085A
    His6-DEP-1 (Cat. #AP11085a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DEP-1 antibody detected the DEP-1 protein (arrow).
    detail
  • IHC-P - His6-DEP-1 Antibody AP11085A
    His6-DEP-1 Antibody (Cat. #AP11085a)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of His6-DEP-1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q12913
Other Accession NP_002834.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 145941 Da
Antigen Region Whole Length of Recombinant DEP-1Protein aa
Additional Information
Gene ID 5795
Other Names Receptor-type tyrosine-protein phosphatase eta, Protein-tyrosine phosphatase eta, R-PTP-eta, Density-enhanced phosphatase 1, DEP-1, HPTP eta, Protein-tyrosine phosphatase receptor type J, R-PTP-J, CD148, PTPRJ, DEP1
Target/Specificity Poly-HIS protein were used to produced this antibody.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHis6-DEP-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTPRJ
Synonyms DEP1
Function Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2 (PubMed:10821867, PubMed:12062403, PubMed:12370829, PubMed:12475979, PubMed:18348712, PubMed:19494114, PubMed:19922411, PubMed:21262971). Plays a role in cell adhesion, migration, proliferation and differentiation (PubMed:12370829, PubMed:14709717, PubMed:16682945, PubMed:19836242). Has a role in megakaryocytes and platelet formation (PubMed:30591527). Involved in vascular development (By similarity). Regulator of macrophage adhesion and spreading (By similarity). Positively affects cell-matrix adhesion (By similarity). Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation (PubMed:16682945). Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR (PubMed:21091576). Positive regulator of endothelial cell survival, as well as of VEGF- induced SRC and AKT activation; through KDR dephosphorylation (PubMed:18936167). Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation (PubMed:19836242). Enhances the barrier function of epithelial junctions during reassembly (PubMed:19332538). Negatively regulates T-cell receptor (TCR) signaling (PubMed:11259588, PubMed:9531590, PubMed:9780142). Upon T-cell TCR activation, it is up- regulated and excluded from the immunological synapses, while upon T- cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling (PubMed:11259588, PubMed:12913111).
Cellular Location Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane. Cell junction Note=After T-cell stimulation, it is temporarily excluded from immunological synapses
Tissue Location Expressed in the promyelocytic cell line HL-60, the granulocyte-macrophage colony-stimulating factor-dependent leukemic cell line F-36P, and the IL3 and erythropoietin-dependent leukemic cell line F-36E. Expressed predominantly in epithelial cells and lymphocytes. Enhanced expression at high cell density
Research Areas
Citations (0)
citation

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Background

May contribute to the mechanism of contact inhibition of cell growth.

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$ 365.00
$ 140.00
Cat# AP11085A
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