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> home > Products > Primary Antibodies > Antibody Collections > Ubiquitin E3 Ligases (RING Domain) Antibodies > RN185 Antibody (Center)
RN185 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | Q96GF1 |
| Other Accession | Q568Y3, Q91YT2, Q6PC78, Q5ZIR9, NP_689480.2 |
| Reactivity | Human, Mouse, Hamster |
| Predicted | Chicken, Zebrafish, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 20459 Da |
| Antigen Region | 88-117 aa |
| Gene ID | 91445 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase RNF185, 632-, RING finger protein 185, RNF185 |
| Target/Specificity | This RN185 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human RN185. |
| Dilution | WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RN185 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RNF185 {ECO:0000303|Ref.1, ECO:0000312|HGNC:HGNC:26783} |
|---|---|
| Function | E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1 (PubMed:21931693). Acts in the endoplasmic reticulum (ER)- associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed:27485036). Protects cells from ER stress-induced apoptosis (PubMed:27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed:24019521). Also acts as a regulator of the innate antiviral response by catalyzing 'Lys-27'-linked polyubiquitination of CGAS at 'Lys-173' and 'Lys-384', thereby promoting CGAS cyclic GMP-AMP synthase activity (PubMed:28273161). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed:24019521). |
| Cellular Location | Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein |
| Tissue Location | Ubiquitously expressed. |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004) :
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$ 365.00
$ 140.00
Cat# AP10818c
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