RNT2 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
---|---|
Primary Accession | O00584 |
Other Accession | NP_003721.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 29481 Da |
Antigen Region | 25-55 aa |
Gene ID | 8635 |
---|---|
Other Names | Ribonuclease T2, 3127-, Ribonuclease 6, RNASET2, RNASE6PL |
Target/Specificity | This RNT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-55 amino acids from the N-terminal region of human RNT2. |
Dilution | WB~~1:500-1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | RNT2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RNASET2 |
---|---|
Synonyms | RNASE6PL |
Function | Ribonuclease that plays an essential role in innate immune response by recognizing and degrading RNAs from microbial pathogens that are subsequently sensed by TLR8 (PubMed:31778653). Cleaves preferentially single-stranded RNA molecules between purine and uridine residues, which critically contributes to the supply of catabolic uridine and the generation of purine-2',3'-cyclophosphate-terminated oligoribonucleotides (PubMed:31778653). In turn, RNase T2 degradation products promote the RNA-dependent activation of TLR8 (PubMed:31778653). Also plays a key role in degradation of mitochondrial RNA and processing of non-coding RNA imported from the cytosol into mitochondria (PubMed:28730546, PubMed:30184494). Participates as well in degradation of mitochondrion-associated cytosolic rRNAs (PubMed:30385512). |
Cellular Location | Secreted. Lysosome lumen. Endoplasmic reticulum lumen. Mitochondrion intermembrane space. Note=Full-length RNASET2 is found in the endoplasmic reticulum while smaller RNASET2 proteolytic products are found in the lysosome fraction. |
Tissue Location | Ubiquitous. Higher expression levels observed in the temporal lobe and fetal brain. |
Provided below are standard protocols that you may find useful for product applications.
Background
This ribonuclease gene is a novel member of the Rh/T2/S-glycoprotein class of extracellular ribonucleases. It is a single copy gene that maps to 6q27, a region associated with human malignancies and chromosomal rearrangement.
References
Fransen, K., et al. Hum. Mol. Genet. 19(17):3482-3488(2010)
Quan, C., et al. Nat. Genet. 42(7):614-618(2010)
Liu, J., et al. J. Cell. Mol. Med. 14(5):1146-1155(2010)
Henneke, M., et al. Nat. Genet. 41(7):773-775(2009)
Monti, L., et al. Oncol. Res. 17(2):69-74(2008)
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.