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NDUFAF1 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NDUFAF1 Antibody (C-term) AP10745B
    NDUFAF1 Antibody (C-term) (Cat. #AP10745b) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the NDUFAF1 antibody detected the NDUFAF1 protein (arrow).
    detail
  • IHC-P - NDUFAF1 Antibody (C-term) AP10745B
    NDUFAF1 antibody (C-term) (Cat. #AP10745b) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUFAF1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9Y375
Other Accession NP_057097.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 37764 Da
Antigen Region 247-276 aa
Additional Information
Gene ID 51103
Other Names Complex I intermediate-associated protein 30, mitochondrial, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1, NDUFAF1, CIA30
Target/Specificity This NDUFAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-276 amino acids from the C-terminal region of human NDUFAF1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUFAF1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFAF1 (HGNC:18828)
Synonyms CIA30
Function As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
Cellular Location Mitochondrion. Mitochondrion matrix. Note=Peripherally associated with the matrix face of the mitochondrial inner membrane
Tissue Location Ubiquitous..
Research Areas
Citations (0)
citation

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Background

The NDUFAF1 gene encodes the human homolog of a Neurospora crassa Cia30 protein involved in the assembly of complex I.

References

Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009) :
Dunning, C.J., et al. EMBO J. 26(13):3227-3237(2007)
Vogel, R.O., et al. Mol. Genet. Metab. 91(2):176-182(2007)
Vogel, R.O., et al. Genes Dev. 21(5):615-624(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

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$ 182.50
$ 70.00
Cat# AP10745B
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