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SERPINA6 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - SERPINA6 Antibody (Center) AP10736c
    Confocal immunofluorescent analysis of SERPINA6 Antibody (Center) (Cat#AP10736c) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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  • WB - SERPINA6 Antibody (Center) AP10736c
    Anti-SERPINA6 Antibody (Center) at 1:1000 dilution + Human plasma tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - SERPINA6 Antibody (Center) AP10736c
    All lanes : Anti-SERPINA6 Antibody (Center) at 1:1000 dilution Lane 1: T47D whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - SERPINA6 Antibody (Center) AP10736c
    Immunohistochemical analysis of AP10736C on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P - SERPINA6 Antibody (Center) AP10736c
    Immunohistochemical analysis of AP10736C on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P - SERPINA6 Antibody (Center) AP10736c
    Immunohistochemical analysis of AP10736C on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, WB, E
Primary Accession P08185
Other Accession NP_001747.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 45141 Da
Antigen Region 197-225 aa
Additional Information
Gene ID 866
Other Names Corticosteroid-binding globulin, CBG, Serpin A6, Transcortin, SERPINA6, CBG
Target/Specificity This SERPINA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197-225 amino acids from the Central region of human SERPINA6.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSERPINA6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SERPINA6
Synonyms CBG
Function Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.
Cellular Location Secreted.
Tissue Location Plasma; synthesized in liver. Has also been identified in a number of glycocorticoid responsive cells
Research Areas
Citations (0)
citation

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Background

This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events.

References

Bailey, S.D., et al. Diabetes Care (2010) In press :
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Holliday, K.L., et al. J Psychosom Res 68(5):469-474(2010)
Braun, B.C., et al. J. Steroid Biochem. Mol. Biol. 120(1):30-37(2010)
Lewis, J.G., et al. Horm. Metab. Res. 42(4):274-279(2010)

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$ 365.00
$ 140.00
Cat# AP10736c
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