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FOLR1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FOLR1 Antibody (N-term) AP10693a
    Western blot analysis of lysate from Hela cell line, using FOLR1 Antibody (N-term)(Cat. #AP10693a). AP10693a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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  • WB - FOLR1 Antibody (N-term) AP10693a
    All lanes : Anti-FOLR1 Antibody (N-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P-Leica - FOLR1 Antibody (N-term) AP10693a
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using AP10693A performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • FC - FOLR1 Antibody (N-term) AP10693a
    Overlay histogram showing HeLa cells stained with AP10693A(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10693A, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P-Leica, E
Primary Accession P15328
Other Accession O15254, NP_000793.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 29819 Da
Antigen Region 33-68 aa
Additional Information
Gene ID 2348
Other Names Folate receptor alpha, FR-alpha, Adult folate-binding protein, FBP, Folate receptor 1, Folate receptor, adult, KB cells FBP, Ovarian tumor-associated antigen MOv18, FOLR1, FOLR
Target/Specificity This FOLR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-68 amino acids from the N-terminal region of human FOLR1.
Dilution WB~~1:2000
IHC-P-Leica~~1:1000
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFOLR1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOLR1
Synonyms FOLR
Function Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed:19074442, PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Has high affinity for folate and folic acid analogs at neutral pH (PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (PubMed:8567728). Required for normal embryonic development and normal cell proliferation (By similarity).
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor Apical cell membrane; Lipid-anchor, GPI- anchor Basolateral cell membrane; Lipid-anchor, GPI-like-anchor. Secreted Cytoplasmic vesicle. Cytoplasmic vesicle, clathrin-coated vesicle. Endosome. Note=Endocytosed into cytoplasmic vesicles and then recycled to the cell membrane
Tissue Location Primarily expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. Detected in placenta and thymus epithelium.
Citations (0)
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Background

The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene.

References

Sivakumaran, S., et al. J. Steroid Biochem. Mol. Biol. 122(5):333-340(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
O'Byrne, M.R., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(8):689-694(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Elwood, P.C., et al. Biochemistry 36(6):1467-1478(1997)

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$ 365.00
$ 140.00
Cat# AP10693a
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