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CCNT1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CCNT1 Antibody (Center) AP10669c
    All lanes : Anti-CCNT1 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: K562 whole cell lysate Lane 6: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 81 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - CCNT1 Antibody (Center) AP10669c
    CCNT1 Antibody (Center) (Cat. #AP10669c) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the CCNT1 antibody detected the CCNT1 protein (arrow).
    detail
  • WB - CCNT1 Antibody (Center) AP10669c
    CCNT1 Antibody (Center) (Cat. #AP10669c) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CCNT1 antibody detected the CCNT1 protein (arrow).
    detail
  • FC - CCNT1 Antibody (Center) AP10669c
    CCNT1 Antibody (Center) (Cat. #AP10669c) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS: 1
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession O60563
Other Accession NP_001231
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 80685 Da
Antigen Region 253-281 aa
Additional Information
Gene ID 904
Other Names Cyclin-T1, CycT1, Cyclin-T, CCNT1
Target/Specificity This CCNT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the Central region of human CCNT1.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCNT1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCNT1
Function Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II) (PubMed:16109376, PubMed:16109377, PubMed:30134174, PubMed:35393539). Required to activate the protein kinase activity of CDK9: acts by mediating formation of liquid-liquid phase separation (LLPS) that enhances binding of P-TEFb to the CTD of RNA Pol II (PubMed:29849146, PubMed:35393539).
Cellular Location Nucleus
Tissue Location Ubiquitously expressed.
Research Areas
Citations ( 0 )

Background

CCNT1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit.

References

Moiola, C., et al. Cell Cycle 9(15):3119-3126(2010)
Schonichen, A., et al. Biochemistry 49(14):3083-3091(2010)
Czudnochowski, N., et al. J. Mol. Biol. 395(1):28-41(2010)
Kapasi, A.J., et al. J. Virol. 83(11):5904-5917(2009)
Cho, S., et al. EMBO J. 28(10):1407-1417(2009)

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$ 365.00
$ 140.00
Cat# AP10669c
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