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AIF1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P55008 |
| Other Accession | NP_001614.3 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 16703 Da |
| Antigen Region | 6-36 aa |
| Gene ID | 199 |
|---|---|
| Other Names | Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, AIF1, G1, IBA1 |
| Target/Specificity | This AIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-36 amino acids from the N-terminal region of human AIF1. |
| Dilution | WB~~1:2000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | AIF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | AIF1 |
|---|---|
| Synonyms | G1, IBA1 |
| Function | Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. |
| Cellular Location | Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:O70200}. Cell projection, ruffle membrane {ECO:0000250|UniProtKB:O70200}; Peripheral membrane protein {ECO:0000250|UniProtKB:O70200}; Cytoplasmic side {ECO:0000250|UniProtKB:O70200}. Cell projection, phagocytic cup {ECO:0000250|UniProtKB:O70200}. Note=Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis {ECO:0000250|UniProtKB:O70200} |
| Tissue Location | Detected in T-lymphocytes and peripheral blood mononuclear cells. |
Provided below are standard protocols that you may find useful for product applications.
Background
AIF1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.
References
Clancy, R.M., et al. Arthritis Rheum. 62(11):3415-3424(2010)
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Jia, J., et al. Pediatr. Res. 67(1):29-34(2010)
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :
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