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TFAM Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, FC, E |
|---|---|
| Primary Accession | Q00059 |
| Other Accession | NP_003192.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 29097 Da |
| Antigen Region | 216-246 aa |
| Gene ID | 7019 |
|---|---|
| Other Names | Transcription factor A, mitochondrial, mtTFA, Mitochondrial transcription factor 1, MtTF1, Transcription factor 6, TCF-6, Transcription factor 6-like 2, TFAM, TCF6, TCF6L2 |
| Target/Specificity | This TFAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the C-terminal region of human TFAM. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 IF~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TFAM Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TFAM (HGNC:11741) |
|---|---|
| Synonyms | TCF6, TCF6L2 |
| Function | Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed:29445193, PubMed:32183942). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:20410300). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed:22037172). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed:22037172). Is able to unwind DNA (PubMed:22037172). Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (PubMed:1737790). Required for maintenance of normal levels of mitochondrial DNA (PubMed:19304746, PubMed:22841477). May play a role in organizing and compacting mitochondrial DNA (PubMed:22037171). |
| Cellular Location | Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells.
References
Wang, W., et al. Nucleic Acids Res. (2010) In press :
Corneveaux, J.J., et al. Hum. Mol. Genet. 19(16):3295-3301(2010)
Akhmetov, I.I., et al. Fiziol Cheloveka 36(2):121-125(2010)
Shulman, J.M., et al. PLoS ONE 5 (6), E11244 (2010) :
Laumet, G., et al. J. Alzheimers Dis. 20(4):1181-1188(2010)
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