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PEMT Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PEMT Antibody (C-term) AP1025B
    All lanes : Anti-PEMT Antibody (C-term) at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: Mouse liver tissue lysate Lane 4: Mouse ovary tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - PEMT Antibody (C-term) AP1025B
    Immunohistochemical analysis of AP1025B on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P - PEMT Antibody (C-term) AP1025B
    Immunohistochemical analysis of AP1025B on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9UBM1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 22134 Da
Antigen Region 170-199 aa
Additional Information
Gene ID 10400
Other Names Phosphatidylethanolamine N-methyltransferase, PEAMT, PEMT, PEMT2, PEMT, PEMPT, PNMT
Target/Specificity This PEMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-199 amino acids from the C-terminal region of human PEMT.
Dilution WB~~1:1000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPEMT Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PEMT {ECO:0000255|HAMAP-Rule:MF_03216}
Synonyms PEMPT, PNMT
Function Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PubMed:12431977, PubMed:15927961). Uses S-adenosylmethionine (S-adenosyl-L-methionine, SAM or AdoMet) as the methyl group donor for the methylation of phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE) to phosphatidylmonomethylethanolamine (1,2-diacyl-sn-glycero-3- phospho-N-methylethanolamine, PMME), PMME to phosphatidyldimethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N,N- dimethylethanolamine, PDME), and PDME to phosphatidylcholine (1,2- diacyl-sn-glycero-3-phosphocholine, PC), producing S-adenosyl-L- homocysteine in each step (PubMed:12431977, PubMed:15927961). Responsible for approximately 30% of hepatic PC with the CDP-choline pathway accounting for the other 70% (Probable).
Cellular Location Endoplasmic reticulum. Note=localized in the endoplasmic reticulum (ER) of the liver and in a lipid metabolism-rich region of the ER known as mitochondria-associated membranes (PubMed:15927961) Adopts a topography within the ER membrane that positions both termini in the cytosol (PubMed:12431977). [Isoform 2]: Endoplasmic reticulum membrane; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03216}
Tissue Location Primarily expressed in liver (at protein level).
Research Areas
Citations (0)
citation

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Background

PEMT is an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17.

References

Walkey C.J., Biochim. Biophys. Acta 1436:405-412(1999).
Shields D.J., Biochim. Biophys. Acta 1532:105-114(2001).
Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).

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$ 182.50
$ 70.00
Cat# AP1025B
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