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PEMT Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q9UBM1 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 22134 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 10400 |
|---|---|
| Other Names | Phosphatidylethanolamine N-methyltransferase, PEAMT, PEMT, PEMT2, PEMT, PEMPT, PNMT |
| Target/Specificity | This PEMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PEMT. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PEMT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PEMT {ECO:0000255|HAMAP-Rule:MF_03216} |
|---|---|
| Synonyms | PEMPT, PNMT |
| Function | Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PubMed:12431977, PubMed:15927961). Uses S-adenosylmethionine (S-adenosyl-L-methionine, SAM or AdoMet) as the methyl group donor for the methylation of phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE) to phosphatidylmonomethylethanolamine (1,2-diacyl-sn-glycero-3- phospho-N-methylethanolamine, PMME), PMME to phosphatidyldimethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N,N- dimethylethanolamine, PDME), and PDME to phosphatidylcholine (1,2- diacyl-sn-glycero-3-phosphocholine, PC), producing S-adenosyl-L- homocysteine in each step (PubMed:12431977, PubMed:15927961). Responsible for approximately 30% of hepatic PC with the CDP-choline pathway accounting for the other 70% (Probable). |
| Cellular Location | Endoplasmic reticulum. Note=localized in the endoplasmic reticulum (ER) of the liver and in a lipid metabolism-rich region of the ER known as mitochondria-associated membranes (PubMed:15927961) Adopts a topography within the ER membrane that positions both termini in the cytosol (PubMed:12431977). [Isoform 2]: Endoplasmic reticulum membrane; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03216} |
| Tissue Location | Primarily expressed in liver (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Alternate splicing of this gene results in three transcript variants encoding two different isoforms.
References
Walkey C.J., Biochim. Biophys. Acta 1436:405-412(1999).
Shields D.J., Biochim. Biophys. Acta 1532:105-114(2001).
Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
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