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FGF9 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FGF9 Antibody (N-term) AP10213A
    FGF9 Antibody (N-term) (Cat. #AP10213a) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the FGF9 antibody detected the FGF9 protein (arrow).
    detail
  • WB - FGF9 Antibody (N-term) AP10213A
    Western blot analysis of FGF9 (arrow) using rabbit polyclonal FGF9 Antibody (N-term) (Cat. #AP10213a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FGF9 gene.
    detail
  • FC - FGF9 Antibody (N-term) AP10213A
    FGF9 Antibody (N-term) (Cat. #AP10213a) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P31371
Other Accession P36364, Q95L12, P54130, NP_002001.1
Reactivity Human, Mouse
Predicted Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 23441 Da
Antigen Region 31-58 aa
Additional Information
Gene ID 2254
Other Names Fibroblast growth factor 9, FGF-9, Glia-activating factor, GAF, Heparin-binding growth factor 9, HBGF-9, FGF9
Target/Specificity This FGF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-58 amino acids from the N-terminal region of human FGF9.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFGF9 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FGF9
Function Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
Cellular Location Secreted.
Tissue Location Glial cells.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis.

References

Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010) :
Kalinina, J., et al. Mol. Cell. Biol. 29(17):4663-4678(2009)
Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009)
Wu, X.L., et al. Am. J. Hum. Genet. 85(1):53-63(2009)

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$ 365.00
$ 140.00
Cat# AP10213A
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