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MAF1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | Q9H063 |
| Other Accession | Q5XIH0, Q9D0U6, A5D9C6, NP_115648.2 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 28771 Da |
| Antigen Region | 90-117 aa |
| Gene ID | 84232 |
|---|---|
| Other Names | Repressor of RNA polymerase III transcription MAF1 homolog, MAF1 |
| Target/Specificity | This MAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-117 amino acids from the Central region of human MAF1. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MAF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MAF1 |
|---|---|
| Function | Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance (PubMed:18377933, PubMed:20233713, PubMed:20516213, PubMed:20543138). Also plays a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation (By similarity). Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB (PubMed:17505538, PubMed:20887893). When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout (PubMed:26941251). Additionally, may also regulate RNA polymerase I and RNA polymerase II- dependent transcription through its ability to regulate expression of the central initiation factor TBP (PubMed:17499043). |
| Cellular Location | Nucleus. Cytoplasm |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression.
References
Kantidakis, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(26):11823-11828(2010)
Shor, B., et al. J. Biol. Chem. 285(20):15380-15392(2010)
Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Rollins, J., et al. Int. J. Biol. Sci. 3(5):292-302(2007)
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