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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ADH4 Antibody (C-term)   

ADH4 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ADH4 Antibody (C-term) AP10128b
    ADH4 Antibody (C-term) (Cat. #AP10128b) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ADH4 antibody detected the ADH4 protein (arrow).
    detail
  • WB - ADH4 Antibody (C-term) AP10128b
    Western blot analysis of ADH4 (arrow) using rabbit polyclonal ADH4 Antibody (C-term) (Cat. #AP10128b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH4 gene.
    detail
  • IHC-P - ADH4 Antibody (C-term) AP10128b
    ADH4 antibody(C-term) (Cat. #AP10128b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH4 antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - ADH4 Antibody (C-term) AP10128b
    ADH4 Antibody (C-term) (Cat. #AP10128b) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS: 3
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P08319
Other Accession NP_000661.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 40222 Da
Antigen Region 319-348 aa
Additional Information
Gene ID 127
Other Names Alcohol dehydrogenase 4, Alcohol dehydrogenase class II pi chain, ADH4
Target/Specificity This ADH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-348 amino acids from the C-terminal region of human ADH4.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADH4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADH4 (HGNC:252)
Function Catalyzes the NAD-dependent oxidation of either all-trans- retinol or 9-cis-retinol (PubMed:17279314). Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate (PubMed:16081420). Also catalyzes the reduction of benzoquinones (PubMed:10514444).
Cellular Location Cytoplasm.
Research Areas
Citations ( 0 )

Background

This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.

References

Pochareddy, S., et al. Gene 460 (1-2), 1-7 (2010) :
Preuss, U.W., et al. Addict Biol (2010) In press :
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Rainero, I., et al. Headache 50(1):92-98(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :

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$ 182.50
$ 70.00
Cat# AP10128b
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