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CD3G

Purified Mouse Monoclonal Antibody

     
  • E - CD3G AO2738a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - CD3G AO2738a
    Figure 2:Western blot analysis using CD3G mAb against human CD3G (AA: extra 23-116) recombinant protein. (Expected MW is 36.6 kDa)
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  • WB - CD3G AO2738a
    Figure 3:Western blot analysis using CD3G mAb against HEK293 (1) and CD3G (AA: extra 23-116)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • WB - CD3G AO2738a
    Figure 4:Western blot analysis using CD3G mouse mAb against Jurkat (1) cell lysate.
    detail
  •  - CD3G AO2738a
    Figure 5:Flow cytometric analysis of Raji cells using CD3G mouse mAb (green) and negative control (red).
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  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P09693
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5B7B2
Isotype Mouse IgG1
Calculated MW 20.5kDa
Immunogen Purified recombinant fragment of human CD3G (AA: extra 23-116) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 917
Other Names T3G; IMD17; CD3-GAMMA
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD3G is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD3G
Synonyms T3G
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR- mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed:8187769). Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.
Cellular Location Cell membrane; Single-pass type I membrane protein
Research Areas
Citations (0)
citation

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References

1.Scand J Immunol. 2014 Nov;80(5):354-61.2.DNA Cell Biol. 2012 Sep;31(9):1480-5.

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$ 385.00
Cat# AO2738a
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