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CD154

Purified Mouse Monoclonal Antibody

     
  • E - CD154 AO2711a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - CD154 AO2711a
    Figure 2:Western blot analysis using CD154 mAb against human CD154 (AA: extra 47-261) recombinant protein. (Expected MW is 50 kDa)
    detail
  • WB - CD154 AO2711a
    Figure 3:Western blot analysis using CD154 mAb against HEK293 (1) and CD154 (AA: extra 47-261)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  •  - CD154 AO2711a
    Figure 4:Flow cytometric analysis of Ramos cells using CD154 mouse mAb (green) and negative control (red).
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P29965
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5A3A9
Isotype Mouse IgG2a
Calculated MW 29.3kDa
Immunogen Purified recombinant fragment of human CD154 (AA: extra 47-261) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 959
Other Names CD40LG; IGM; IMD3; TRAP; gp39; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD154 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD40LG
Synonyms CD40L, TNFSF5, TRAP
Function Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).
Cellular Location Cell membrane; Single-pass type II membrane protein. Cell surface
Tissue Location Specifically expressed on activated CD4+ T- lymphocytes
Research Areas
Citations (0)
citation

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References

1.Hematology. 2016 Apr;21(3):187-92. 2.Mol Cell Probes. 2015 Dec;29(6):335-342.

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$ 385.00
Cat# AO2711a
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