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COVID19
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CD209

Purified Mouse Monoclonal Antibody

     
  • E - CD209 AO2686a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - CD209 AO2686a
    Figure 2:Western blot analysis using CD209 mAb against human CD209 (AA: extra 270-404) recombinant protein. (Expected MW is 41.1 kDa)
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  • WB - CD209 AO2686a
    Figure 3:Western blot analysis using CD209 mAb against HEK293 (1) and CD209 (AA: extra 270-404)-hIgGFc transfected HEK293 (2) cell lysate.
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  • WB - CD209 AO2686a
    Figure 4:Western blot analysis using CD209 mouse mAb against THP-1 (1), HL-60 (2), and A431 (3) cell lysate.
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  •  - CD209 AO2686a
    Figure 5:Flow cytometric analysis of Ramos cells using CD209 mouse mAb (green) and negative control (red).
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession Q9NNX6
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5C2G9
Isotype Mouse IgG1
Calculated MW 45.8kDa
Immunogen Purified recombinant fragment of human CD209 (AA: extra 270-404) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 30835
Other Names CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD209 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD209
Synonyms CLEC4L
Function Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response.
Cellular Location [Isoform 1]: Cell membrane; Single- pass type II membrane protein [Isoform 3]: Cell membrane; Single- pass type II membrane protein [Isoform 5]: Cell membrane; Single- pass type II membrane protein [Isoform 7]: Secreted. [Isoform 9]: Secreted. [Isoform 11]: Secreted.
Tissue Location Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.
Research Areas
Citations (0)
citation

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References

1.Blood. 2015 Oct 15;126(16):1911-20. 2.PLoS One. 2014 Aug 22;9(8):e105236.

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$ 385.00
Cat# AO2686a
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