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CD10

Purified Mouse Monoclonal Antibody

     
  • E - CD10 AO2671a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - CD10 AO2671a
    Figure 2:Western blot analysis using CD10 mAb against human CD10 (AA: extra 549-750) recombinant protein. (Expected MW is 48.8 kDa)
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  • WB - CD10 AO2671a
    Figure 3:Western blot analysis using CD10 mAb against HEK293 (1) and CD10 (AA: extra 549-750)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  •  - CD10 AO2671a
    Figure 4:Flow cytometric analysis of HL-60 cells using CD10 mouse mAb (green) and negative control (red).
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  • IHC - CD10 AO2671a
    Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD10 mouse mAb with DAB staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P08473
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3G6B11
Isotype Mouse IgG1
Calculated MW 85.5kDa
Immunogen Purified recombinant fragment of human CD10 (AA: extra 549-750) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4311
Other Names NEP; SFE; MME; CALLA; CMT2T; SCA43
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD10 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MME {ECO:0000303|PubMed:27588448, ECO:0000312|HGNC:HGNC:7154}
Function Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:6208535, PubMed:6349683, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991, PubMed:6349683). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed:6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675, PubMed:6349683). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed:16254193, PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).
Cellular Location Cell membrane; Single-pass type II membrane protein
Research Areas
Citations (0)
citation

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References

1.Pathobiology. 2015;82(6):259-63. 2.Asian Pac J Cancer Prev. 2015;16(8):3147-52.

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$ 385.00
Cat# AO2671a
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