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Dynamin-2

Purified Mouse Monoclonal Antibody

     
  • E - Dynamin-2 AO2665a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - Dynamin-2 AO2665a
    Figure 2:Western blot analysis using Dynamin-2 mAb against human Dynamin-2 (AA: 520-744) recombinant protein. (Expected MW is 28 kDa)
    detail
  • WB - Dynamin-2 AO2665a
    Figure 3:Western blot analysis using Dynamin-2 mAb against HEK293 (1) and Dynamin-2 (AA: 520-744)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • WB - Dynamin-2 AO2665a
    Figure 4:Western blot analysis using Dynamin-2 mouse mAb against U251 (1), Hela (2), and K562 (3) cell lysate.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P50570
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3F5C7
Isotype Mouse IgG2a
Calculated MW 98kDa
Immunogen Purified recombinant fragment of human Dynamin-2 (AA: 520-744) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1785
Other Names DNM2;DYN2; CMT2M; DYNII; LCCS5; CMTDI1; CMTDIB; DI-CMTB
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDynamin-2 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNM2 (HGNC:2974)
Synonyms DYN2
Function Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission at plasma membrane during endocytosis and filament remodeling at many actin structures during organization of the actin cytoskeleton (PubMed:15731758, PubMed:19605363, PubMed:19623537, PubMed:33713620, PubMed:34744632). Plays an important role in vesicular trafficking processes, namely clathrin-mediated endocytosis (CME), exocytic and clathrin-coated vesicle from the trans-Golgi network, and PDGF stimulated macropinocytosis (PubMed:15731758, PubMed:19623537, PubMed:33713620). During vesicular trafficking process, associates to the membrane, through lipid binding, and self-assembles into ring-like structure through oligomerization to form a helical polymer around the vesicle membrane and leading to vesicle scission (PubMed:17636067, PubMed:34744632, PubMed:36445308). Plays a role in organization of the actin cytoskeleton by mediating arrangement of stress fibers and actin bundles in podocytes (By similarity). During organization of the actin cytoskeleton, self-assembles into ring-like structure that directly bundles actin filaments to form typical membrane tubules decorated with dynamin spiral polymers (By similarity). Self-assembly increases GTPase activity and the GTP hydrolysis causes the rapid depolymerization of dynamin spiral polymers, and results in dispersion of actin bundles (By similarity). Remodels, through its interaction with CTTN, bundled actin filaments in a GTPase-dependent manner and plays a role in orchestrating the global actomyosin cytoskeleton (PubMed:19605363). The interaction with CTTN stabilizes the interaction of DNM2 and actin filaments and stimulates the intrinsic GTPase activity that results in actin filament-barbed ends and increases the sensitivity of filaments in bundles to the actin depolymerizing factor, CFL1 (By similarity). Plays a role in the autophagy process, by participating in the formation of ATG9A vesicles destined for the autophagosomes through its interaction with SNX18 (PubMed:29437695), by mediating recycling endosome scission leading to autophagosome release through MAP1LC3B interaction (PubMed:29437695, PubMed:32315611). Also regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane (By similarity). Also plays a role in cytokinesis (By similarity). May participate in centrosome cohesion through its interaction with TUBG1 (By similarity). Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Involved in membrane tubulation (PubMed:24135484).
Cellular Location Cytoplasm, cytoskeleton. Cytoplasmic vesicle, clathrin-coated vesicle. Cell projection, uropodium. Endosome Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Recycling endosome. Cell projection, phagocytic cup {ECO:0000250|UniProtKB:P39054}. Cytoplasmic vesicle, phagosome membrane {ECO:0000250|UniProtKB:P39054}; Peripheral membrane protein {ECO:0000250|UniProtKB:P39054}. Cell projection, podosome {ECO:0000250|UniProtKB:P39054}. Cytoplasm {ECO:0000250|UniProtKB:P39052}. Cell junction {ECO:0000250|UniProtKB:P39052}. Postsynaptic density {ECO:0000250|UniProtKB:P39052}. Synapse, synaptosome {ECO:0000250|UniProtKB:P39052}. Midbody {ECO:0000250|UniProtKB:P39052} Membrane, clathrin-coated pit {ECO:0000250|UniProtKB:P39052} Note=Localized in recycling endosomes fragment to release nascent autophagosomes (PubMed:32315611). Co-localizes with PIK3C3 and RAB5A to the nascent phagosome. Localized at focal ahesion site upon induction of focal adhesions and stress-fiber formation, when interacts with SDC4 (By similarity). Exists as a dynamic component of the centrosome Associates with clathrin-coated vesicles at both the plasma membrane and the trans-Golgi network (TGN) (By similarity) {ECO:0000250|UniProtKB:P39052, ECO:0000250|UniProtKB:P39054, ECO:0000269|PubMed:32315611}
Tissue Location Widely expressed (PubMed:7590285). Expressed in skeletal muscle and the peripheral nerve (PubMed:19623537)
Research Areas
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References

1.Cancer Med. 2014 Feb;3(1):14-24.2.Eur J Obstet Gynecol Reprod Biol. 2012 Oct;164(2):180-4.

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$ 385.00
Cat# AO2665a
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