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CD195

Purified Mouse Monoclonal Antibody

     
  • E - CD195 AO2654a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - CD195 AO2654a
    Figure 2:Western blot analysis using CD195 mAb against human CD195 (AA: extra mix) recombinant protein. (Expected MW is 36.7 kDa)
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  • WB - CD195 AO2654a
    Figure 3:Western blot analysis using CD195 mAb against HEK293 (1) and CD195 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
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  • WB - CD195 AO2654a
    Figure 4:Western blot analysis using CD195 mouse mAb against L-02 (1) cell lysate.
    detail
  •  - CD195 AO2654a
    Figure 5:Flow cytometric analysis of HL-60 cells using CD195 mouse mAb (green) and negative control (red).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P51681
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6H3D5
Isotype Mouse IgG1
Calculated MW 40.5kDa
Immunogen Purified recombinant fragment of human CD195 (AA: extra mix) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1234
Other Names CCR5; CKR5; CCR-5; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD195 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCR5 (HGNC:1606)
Synonyms CMKBR5
Function Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Participates in T-lymphocyte migration to the infection site by acting as a chemotactic receptor (PubMed:30713770).
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1a and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
Research Areas
Citations (0)
citation

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References

1.J Leukoc Biol. 2015 Jul;98(1):59-71. 2.J Gen Virol. 2015 Aug;96(8):2074-8.

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$ 385.00
Cat# AO2654a
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