CD147
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, ICC, E |
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Primary Accession | P35613 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 7H4D4 |
Isotype | Mouse IgG1 |
Calculated MW | 42.2kDa |
Immunogen | Purified recombinant fragment of human CD147 (AA: extra 138-323) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Gene ID | 682 |
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Other Names | BSG; OK; 5F7; TCSF; EMMPRIN |
Dilution | E~~ 1/10000 WB~~ 1/500 - 1/2000 IHC~~ 1/200 - 1/1000 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | CD147 is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | BSG (HGNC:1116) |
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Function | [Isoform 1]: Essential for normal retinal maturation and development (By similarity). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687). May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed:21620857). |
Cellular Location | Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [Isoform 2]: Cell membrane; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P26453}. Endosome Endoplasmic reticulum membrane; Single- pass type I membrane protein {ECO:0000250|UniProtKB:P26453} Basolateral cell membrane; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P26453} [Isoform 4]: Cell membrane; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P26453} |
Tissue Location | [Isoform 1]: Retina-specific (PubMed:25957687). Expressed in retinal cone photoreceptors (at protein level) (PubMed:25957687). [Isoform 3]: Highly expressed in the bone marrow, fetal liver, lung, testis and thymus. |
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Provided below are standard protocols that you may find useful for product applications.
References
1.Oncotarget. 2016 Feb 2;7(5):5613-29.2.J Biochem. 2016 May;159(5):481-90.
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